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nb100 65265  (Novus Biologicals)


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    Structured Review

    Novus Biologicals nb100 65265
    Nb100 65265, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nb100+65265/TCR+alpha+Antibody+(3A8)/pm32815561-36-6-7
    Average 91 stars, based on 1 article reviews
    nb100 65265 - by Bioz Stars, 2026-09
    91/100 stars

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    Incubation:

    Article Title: Lymphocyte predominant cells of nodular lymphocyte predominant Hodgkin lymphoma interact with rosetting T cells in an immunological synapse.
    Article Snippet: Immunohistochemical staining and proximity ligation assay Immunohistochemical staining for CD20 (clone L26, RTU, Agilent-DAKO, Santa Clara, CA, USA), PD1 (clone EP239, RTU, DCS-Diagnostics, Hamburg, Germany), IgD (IR517, RTU, Agilent-DAKO), MHCII alpha chain (mouse monoclonal, 1:500 dilution, HLA-DR, clone TAL1.B5, Agilent-DAKO), MHCII beta chain (rabbit monoclonal, 1:25 dilution, HLA-DPB1, clone SP229, LS-C210442, Lifespan Biosciences, Seattle, WA, USA), TCR alpha chain (mouse monoclonal, 1:100 This article is protected by copyright. .. All rights reserved. dilution, clone 3A8, NB100-65265, Novus Biologicals, Wiesbaden, Germany), PD-L1 (1:100 dilution, ZR3, Zeta Corporation, Arcadia, CA, USA), OCT2 (dilution 1:100, clone MRQ-2, Cell Marque, Merck, Darmstadt, Germany) and CD69 (1:50 dilution, HPA050525, Atlas Antibodies, Sigma-Aldrich, Munich, Germany) was performed in TMA format as previously described.22 In brief, after deparaffinization and antigen demasking at pH 6 or pH 9, slides were incubated with the primary antibody for 2 hours, and visualized using the Envision-FLEX kit (DAKO). .. For double staining of the MHCII beta chain and TCR alpha chain, the TMA was incubated for 2 hours with the anti-MHCII beta chain antibody; after detection with the Permanent HRP Green Kit (ZUC070-100, Zytomed Systems, Berlin, Germany), the TMA was incubated with the anti-TCR alpha chain antibody overnight at 4°C.



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    91
    Novus Biologicals nb100 65265
    Nb100 65265, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nb100+65265/TCR+alpha+Antibody+(3A8)/pm32815561-36-6-7
    Average 91 stars, based on 1 article reviews
    nb100 65265 - by Bioz Stars, 2026-09
    91/100 stars
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